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Image Search Results
Journal: Neuron
Article Title: Enhancer AAV toolbox for accessing and perturbing striatal cell types and circuits
doi: 10.1016/j.neuron.2025.04.035
Figure Lengend Snippet: (A) Enhancer AAVs were individually packaged, pooled, and injected RO into adult mice. (B and C) Example images of two different triple-labeling combinations. AAVs used in (B): AiP12609 (D1 MSN-SYFP2), AiP12700 (D2 MSN-mTFP1), and AiP13738 (cholinergic-tdTomato). AAVs used in (C): AiP12689 (Sst-Chodl-SYFP2), AiP13808 (Pvalb-Pthlh-mTFP1), and AiP13738 (cholinergic-tdTomato). (D and E) Quantification of cell counts in dorsal striatum (D) and cortex (E). Barnard’s test was used to evaluate enhancer expression overlap in cortex versus STRd ( n = 1 mouse and ROI per region). For D1 MSN+D2 MSN+cholinergic, no significant difference was found (two-sided p = 0.8882), while for Sst-Chodl+Pvalb-Pthlh+cholinergic enhancers, a significant difference in enhancer expression overlap between cortex and STRd was observed (two-sided p < 0.0001). (F–H) Single-cell patch-clamp recordings from coronal brain slices derived from a mouse with multiplex injection of AiP15050 (Sst-Chodl-SYFP2), AiP13808 (Pvalb-Pthlh-mTFP1), and AiP13738 (cholinergic-tdTomato). Representative responses to hyperpolarizing and depolarizing current steps recorded from the same mouse (top) with example single action potential traces (middle) and current injection steps (bottom). (I and J) 3D plots comparing electrophysiological features that distinguish the three striatal interneuron types. Diamonds represent the mean values for each interneuron type. Circles with black outline correspond to neuron traces in (F)–(H). PERMANOVA using all five electrophysiological features revealed significant differences across the three cell type populations (F = 51.071, p = 0.001). A pairwise PERMANOVA post hoc analysis indicated significant differences between all cell type pairwise comparisons ( p < 0.01). Number of cells recorded: Pvalb-Pthlh, n = 13; Sst-Chodl, n = 16; cholinergic, n = 8. A total of 7 mice were used for triple-RO injection patch-clamp recording experiments. See also .
Article Snippet:
Techniques: Injection, Labeling, Expressing, Patch Clamp, Derivative Assay, Multiplex Assay